Morphofunctional properties of adipose-derived multipotent mesenchymal stromal cells in vitro in ovariectomized mice of different ages

A. Ustymenko; V. Kyryk; T. Lutsenko; O. Tsupykov; G. Butenko

A. Ustymenko 1State Institute of Genetic and Regenerative Medicine of the National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine 2Chebotarev State Institute of Gerontology of the National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
V. Kyryk 1State Institute of Genetic and Regenerative Medicine of the National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine 2Chebotarev State Institute of Gerontology of the National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
T. Lutsenko 1State Institute of Genetic and Regenerative Medicine of the National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine 2Chebotarev State Institute of Gerontology of the National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
O. Tsupykov State Institute of Genetic and Regenerative Medicine of the National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine; Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
G. Butenko 1State Institute of Genetic and Regenerative Medicine of the National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine 2Chebotarev State Institute of Gerontology of the National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine

Keywords: ovariectomy, adipose-derived mesenchymal stromal cells, directed differentiation of stem cell

Abstract

In modern regenerative medicine, adipose-derived multipotent mesenchymal stromal cells (MMSCs) are particular interest for researchers and clinicians since they are widely used in the treatment of a number of diseases. Given the influence of sex hormones on the function of adipose tissue, a key aspect remains the proper assessment of biological safety and the prediction of the effectiveness of such cell transplants in conditions associated with age-related disorders of ovarian endocrine function.
Purpose: to determine the morphofunctional properties of adipose-derived multipotent mesenchymal stromal cells in vitro in young and old ovariectomized mice.
Materials and methods. Disorders of ovarian endocrine function were reproduced on an in vivo model of ovariectomy in young and old female CBA/Ca mice at the age of 2 and 14 months, respectively. ADSCs were obtained from adipose tissue and phenotyped by flow cytometry. The morphological characteristics, proliferative potential, and ability to direct multilineage differentiation in osteogenic and adipogenic directions in vitro were evaluated.
Results. Under the impact of ovariectomy in young animals, there a decrease in the proliferative and clonogenic potential of ADSCs in vitro was observed, which parameters approach the level of old mice. In addition, the osteogenic potential of ADSCs in vitro was reduced, as well as the enhancement of directed adipogenic differentiation was shown while increasing body weight of animals.
Conclusion. The results of the study indicate the negative impact of ovariectomy at a young age on the functional activity of ADSCs.

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