Effects of human Wharton’s jelly-derived mesenchymal stem cells and Interleukin-10 on behavioural responses of rats with experimental allergic encephalomyelitis
Abstract
On the rat model of experimental allergic encephalomyelitis (EAE), as analogue of multiple sclerosis of human, we studied the effect of Wharton’s jelly-derived mesenchymal stem cells and interleukin-10 on the functional parameters of the CNS.
Materials and methods. EAE was induced with spinal cord homogenate of rats with complete Freund’s adjuvant. MSCs were isolated by the explants technique from Wharton’s jelly of the human umbilical cord and cultured up to two passages. Recombinant IL-10 was administered intravenously on the day 10 after induction of EAE and subocipitally on the day 17 at a dose of 0.2 mg per animal. Cell transplantation performed subocipitally on the day 17 at a dose of 106 cells per animal. Behavioral reactions studied in the “open field” test three times: on the day 12, 15 and 24 after the induction of EAE.
Results. Induced EAE results in significant changes in adaptive behavior of rats on the 12th day as suppression of orienting-exploratory and activation of emotional activity. The application of MSCs and IL-10 has normalized the rates of cognitive activities (orienting-exploratory activity) and emotional sphere (the level of fear, anxiety).
Conclusion. Combined treatment of induced EAE in rats with interleukin-10 and human Wharton’s jelly-derived mesenchymal stem cells is effective at correction of behavioral responses of animals.
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